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KMID : 1004820110120030147
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Journal of Biomedical Research
2011 Volume.12 No. 3 p.147 ~ p.155
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Biomedical Application of PHBV/Collagen (PHCP) Nanofiber as the Wound Dressing
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Guang Xu Zi
Moon Chan-Mi
Kwak Jyung-Sik
Oh Ki-Wan
Bae Han-Ik
Han Jin-Yi
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Abstract
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In this study, a nanofibrous scaffold was obtained by co-electrospinning poly (3-hydroxybutyrate- co-3-hydroxyvalerate) (PHBV) and collagen in 2,2,2-trifluoroethanol at a ratio of 3/7. The fiber diameters were in the range of 250-600 nm. It was found that PHBV/Collagen (PHCP) nanofibrous scaffold showed greater proliferation than the PHBV nanofibrous scaffold induced by oxidant in NIH3T3 cells. Otherwise, in the early-stage wound-healing mouse model, wound closure was evaluated according to wound size reduction and histology of regenerated skin on the backs of mice. Each of the tissues removed on day 0, 3, 6, 9, 12, 15, and 18 was used for analysis of biochemical and pathological changes. None of the nanofiber-attached mice showed significant difference on the third day, however, from the third day until the ninth day, significantly faster healing was observed in PHCP-attached mice, compared to control wounds in epithelialization, wound contraction, and histopathological examinations. These results strongly support the beneficial effects of biomedical application of PHCP nanofiber in acceleration of the initial phase of wound healing through α-SM actin contraction.
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KEYWORD
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PHBV/Collagen (PHCP), Nanofiber, Tissue engineering, Wound healing
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